WebWe aim to research the molecular mechanism of lncRNA NEAT1 in the activation of astrocytes in a cerebral ischemia-reperfusion injury model. Mouse model of cerebral … To assess the functional role of NEAT1 in colorectal cancer cells, first we examined the baseline NEAT1 RNA levels in eight CRC cell lines (RKO, CACO2, SW1116, LOVO, SW480, SW620, HT29, and HCT116) by RT-qPCR (Fig. 2a). NEAT1 was expressed at much higher levels in the HCT116 and SW1116 cells and relatively … See more To determine the effect of altered NEAT1 expression on the migration and invasion of CRC cells, NEAT1-siRNA-transfected and NEAT1 plasmid-transfected CRC cells were wounded by … See more As described above, NEAT1 plays an important role in CRC progression, although the detailed mechanism remains unknown. Because lncRNAs can interact with proteins, a biotin … See more To elucidate whether NEAT1 functioned in CRC cells in a DDX5-mediated manner, we performed CCK-8 and EdU assays. The results showed that overexpression of NEAT1 recovered the … See more Recently, decreased NEAT1 expression was reported to inhibit Wnt/β-catenin signaling pathway activity in glioblastoma [21]. In our study, we … See more
Long Noncoding RNA NEAT1 Promotes Growth and Metastasis of ...
Web8 Apr 2024 · NEAT1 knockdown and NEAT1 overexpression were carried out by synthesization of shRNA that specifically targeted NEAT1 (shNEAT1) and the PCR … Web17 Jul 2024 · In cell apoptosis assay, the shNEAT1-HT29 cells exhibited significantly increased apoptotic rate when compared with cells of negative control (Figure 3(e)). In … gym bald hills
Frontiers Depletion of LncRNA NEAT1 Rescues Mitochondrial …
WebThe Neat1 shRNA or nonsense shRNA was inserted into the BLOCK-iT™ lentiviral RNAi expression system (Invitrogen). The sequences of Neat1 shRNA are listed in Table S1. … Web11 Sep 2024 · Results showed that exo shNEAT1 or miR-23a mimic significantly downregulated expression of lncRNA NEAT1 and MDM2 and extent of p65 … Web20 Mar 2024 · NEAT1 knockdown further decreased cell growth in cisplatin- or taxol-treated cells, demonstrating a synergistic effect of the combination of shNEAT1 and … boys rain boots toddler